To extract oil from wheat bran, using supercritical carbon dioxide (SCO2), the sample was dried and powdered with 500 μm of the size. The supercritical fluid extraction was carried out in a semi-batch flow type process at temperatures ranging from 30-60ºC and pressures ranging from 8-25 MPa for 2 hours. The CO2 flow rate (26.81 g/min) was constant entire the extraction period. Solubility of wheat bran oil by SCO2 as a solvent was measured in all conditions. The highest solubility of oil (0.022g/g CO2) was found at 30 MPa and 60 ºC. The extracted oil was identified by GC for fatty acids components. The amount of palmitic and linoleic acid was high. The highest amount of linoleic acid (44.45%) and palmitic acid (21.45%) was found in wheat bran oil extracted at 30 MPa and 60 oC, respectively. Elaic.oleic, linolenic and butric acid were present significant amount in extracted oil from wheat bran. Tocopherols, an important phytochemical for natural antioxidant in the wheat bran oil extracted by SCO2 was analyzed by HPLC. The highest amount of α-tocopherol (399 μg/g), β- tocopherol (183 μg/g) and γ-tocopherol (312 μg/g) was found in wheat bran oil extracted at 20 MPa, 40ºC and 25 MPa, 30ºC and 20 MPa, 60ºC, respectively.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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