Mannitol, a naturally occurring polyol, is widely used in the food, pharmaceutical, medicine, and chemical industries. Most commercial mannitol has been synthesized chemically, but strong demand for natural products caused many researchers to study the bioconversion process for mannitol production. This study reported the conversion of fructose to mannitol in a packed bed reactor in semi-continuous mode bacterial cells immobilized on the activated carbons. The strain to convert the fructose to mannitol was isolated from kimches and identified. Fermentation was conducted in the immobilized reactor, a 250 x 60 mm glass column with water-cooling jacket, was packed with activated carbons (30 x 80 mesh). Fructose (10% w/v) was pumped with an downward flow of 5 ml/min and the outlet stream was continuously recirculated into a reservoir and back to the column. Five consecutive batches using fresh medium for each one were carried out at 25oC. The highest yield was obtained at the second batch (80%) and decreased gradually (about 60%) as the batches proceeded.
키워드
mannitolbiotransformationLeuconostoc
저자
Jun Taek HONG [ Dept. of Food Science and Biotechnology, Joongbu University ]
Sun Kyun YOO [ Dept. of Food Science and Biotechnology, Joongbu University ]
Sung Paal YIM [ Korea Atomic Energy Research Institute ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
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