Earticle

초록

영어

Ginsenosides as aglycons are physiologically important active compounds. The ginsenosides such as Ra, Rb, Rc, Rd, Rg3 and Rh2 have the same aglycon, but different sugar moieties at C-3 and C-20. As a result, their physiological functions differ much from each other. In recent days, research has shown that ginsenoside being a little in nature could be produced from the structurally different ginsenoside by a bioconversion technique. Most microorganisms used previously for the bioconversion were the bacteria originated from human intestines, and only few fungi were reported. In this report, we describe the bioconversion of ginsenosides using mold, Trichoderma reesei. Transformation flasks were prepared by adding 20 ml of inoculum
from 24 h cultures grown in 500 ml flasks containing 80.0 ml of mineral medium and 1% (v/v) ginsenosides. Microorganism control flasks were prepared by adding 20 ml of inoculum to 500 ml flasks containing 80 ml of mineral medium without substrate. The Substrate control flasks contained 100 ml of mineral medium and 1% (v/v) ginsenosides. The culture flasks were
incubated in a shaker at 30°C and 200 rpm for 24 h. All metabolites were analyzed by TLC plates (silica gel 60 F254 ) pre-coated aluminum sheet (0.2 mm, 20 x 20 cm). The mobile phase consisted of BuOH, Ethyl acetate, water (5:1:4). Densitometry scanning was performed using LJ Color 2840. The intensity of density of spots was evaluated using Scion Image Program. We
found that the cell-free extract of the yeast converted the composition of ginsenosides of red ginseng to other composition of ginsenosides.

키워드

저자

• Joong Hyun YEOM [ Dept. of Food Science and Biotechnology, Joongbu University ]
• Sun Kyun YOO [ Dept. of Food Science and Biotechnology, Joongbu University ]
• Sung Paal YIM [ Dept. of Food Science and Biotechnology, Joongbu University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576