Earticle

초록

영어

Translation initiation, a process which forms an initiation complex by assembly of the ribosomal subunit and initiator tRNA at the start codon on the mRNA, is regarded as a rate-limiting step in protein synthesis. Three initiation factors (IF1, IF2, and IF3) are participated in translation initiation process. IF3 assisted by IF1 promotes the dissociation of vacant 70S subunit to prevent forming premature initiation complex (PIC). IF2 accelerates association of initiator tRNA, 30S subunit, and mRNA. IF2 might play a key role in determining the rate of translation initiation because it affects to the formation of the initiation complex and subsequent passage from the initiation to the
elongation. However, the relationship between the structure of the IF2 and the rate of translation initiation was not revealed. In this study, we engineered chromosomal IF2 encoded by infB using error-prone PCR and Red/ET recombination to modulate the translation rate. Also, the
change of the translation rate caused by engineered IF2 variants was investigated.

키워드

저자

• Byung Eun MIN [ School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology ]
• Sang Woo SEO [ Department of Chemical Engineering, Pohang University of Science and Technology ]
• Gyoo Yeol JUNG [ 1School of Interdisciplinary Bioscience and Bioengineering and Department of Chemical Engineering, Pohang University of Science and Technology ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576