Earticle

초록

영어

Squalene is a triterpenoic hydrocarbon (C30H50). Dietary squalene is able to stimulate nonspecific immune functions and reduce the levels of lowdensity lipoprotein as well as triglyceride. The shark liver oil, the current major natural resource, is limiting and its future supply is uncertain. In this work, Saccharomyces cerevisiae, some strains of which are known to
accumulate a large amount of ergosterol, was metabolically engineered to overproduce squalene. In eukaryotes, isoprenoids are synthesized from acetyl CoA through mevalonate. The catalytic domain of the gene encoding hydroxymethylglutaryl CoA reductase (HMGR), a key regulatory step in mevalonate pathway, was over-expressed under the GAL10 promoter in S.
cerevisiae 2805 strain. Over-expression of HMGR resulted in the intracellular accumulation of squalene (0.49 g/L) in a shake flask experiment. Other genes involved in isoprenoid synthesis such as ispA (a bifunctional synthase for geranyl diphosphate and farnesyl diphosphate),
GGPP (geranylgeranyl diphosphate) synthase or MVAE (acetyl-CoA acetyltransferase) were also overexpressed in S. cerevisiae 2805 strain. Over-expression of these genes alone did not show any improvements in squalene accumulation. Coexpression of ispA with HMGR, however,
dramatically increased the level of squalene (up to 1.8 g/L). The level of squalene accumulation varied among different strains of S. cerevisiae.

키워드

저자

• Hee-Kyoung RYU [ Division of Biomaterials Science, KRIBB, Daejeon 305-806, Korea. ]
• Mi-Ran JO [ Division of Biomaterials Science, KRIBB, Daejeon 305-806, Korea. ]
• Jake WHANG [ Division of Biomaterials Science, KRIBB, Daejeon 305-806, Korea. ]
• Seon-Won KIM [ Gyeongsang National University ]
• Eui-Sung CHOI [ Division of Biomaterials Science, KRIBB, Daejeon 305-806, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576