Asad Ali SHAH, Chong-Long WANG, Sook-Hee LEE, Jung-Hun KIM, Hee-Jeong JANG, Jong-Wook SONG, Seo-Hee KANG, Eui-Sung CHOI, Seon-Won KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A115111
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원문정보
초록
영어
Due to limiting fuel reservoirs, high oil prices and politico-strategic problems it is urging to produce biofuels as transportation energy. Fossil fuel is a complex mixture of hydrocarbons including linear, branched, and cyclic alkanes, and aromatics. Terpenoid based biofuel is promising alternative to replace the present hydrocarbon fuels. The terpenoid biosynthesis pathways could produce branched-chain and cyclic alkanes, alkenes, and alcohols with desired chain lengths. But their mass production is hampered due to their toxicity to host strain, E. coli. So it is very pressing to develop tolerant host strain to noxious terpenoid biofuels.In our study we have over expressed different stress responsive genes (marA, cls, imp ,and cti) which are suppose to provide tolerance against hostile solvents. Plate overlay, broth overlay, and viable cell count and gas chromatography analysis were conducted to observe any improved tolerance by the tested genes. In present work hexane is used as dilution solvent of terpenoids. Overexpression of marA increased tolerance of E. coli to geraniol, farnesol, and myrcene but showed no effect on isopentenol. Mutant strain with marA deletion was much more sensitive to the terpenoids, compared to its wild type strain. This work was supported by the 21C Frontier Microbial Genomics and Applications Center Program, EB-NCRC (Grant No. R15-2003-012-02001-0), and BK21 program of Korea.
키워드
Biofuelterpenoidssolvent tolerance
저자
Asad Ali SHAH [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Chong-Long WANG [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Sook-Hee LEE [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Jung-Hun KIM [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Hee-Jeong JANG [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Jong-Wook SONG [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Seo-Hee KANG [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Eui-Sung CHOI [ Systems Microbiology Research Center, KRIBB ]
Seon-Won KIM [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동