Earticle

초록

영어

Comparative proteome analysis has been carried out to elucidate the physiological and metabolic changes responsible for succinic acid overproduction and cell growth. The proteome of the metabolically engineered succinic acidoverproducing bacterium, Mannheimia succiniciproducens LPK7, were compared with that of the wild type strain (MBEL55E). ATP synthesis, pyruvate metabolism, glycolysis, and amino acid biosynthesis of the LPK7 strain were much changed in expression level. Based on the proteome changes, we could reduce the formation of pyruvic acid, a newly formed byproduct in the LPK7 strain, by adding pantothenate and L-cysteine, serving as precursors of CoA biosynthesis, to the medium. These results clearly suggest that further comparative proteome profiling under various genetic and/or environmental perturbations will reveal more new insights into the physiology and metabolic
characteristics and, therefore, will be used to develop metabolic engineering strategies. [This work was supported by the Genome-Based Integrated Bioprocess Development Project of the Ministry of Education, Science and Technology (MEST). Further supports by the LG Chem Chair Professorship, Microsoft, IBM SUR, WCU (World Class University) program through the
National Research Foundation of Korea funded by MEST (R32-2008-000-10142-0) are ppreciated.]

키워드

저자

• Eun Jung CHOI [ Dept. of Chemical and Biomolecular Engineering, KAIST ]
• Jeong Wook LEE [ Dept. of Chemical and Biomolecular Engineering, KAIST ]
• Hyohak SONG [ Dept. of Chemical and Biomolecular Engineering, KAIST ]
• Jong Shin YOO [ Dept. of Chemical and Biomolecular Engineering, KAIST ]
• Sang Yup LEE [ Dept. of Chemical and Biomolecular Engineering, KAIST ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576