Earticle

초록

영어

Tylosin is a 16-membered macrolide antibiotics produced by Streptomyces fradiae, composed of a branched lactone ring, tylactone and three deoxysugars, mycinose, mycaminose and mycarose. Of these different deoxysugars, mycinose is attached to C-23 position of tylactone. In
this study, the mycinose biosynthetic pathway was identified through the heterologous expression of several gene sets in an engineered strain of Streptomyces venezuelae. A plasmid harboring TDPdeoxyallose biosynthetic gene set (tylD-tylJ-tylN-desIII-desIV) was
constructed and introduced into the S. venezuelae YJ028 bearing a deletion of native pikromycin polyketide synthase and desosamine gene cluster. As a result, deoxyallosyl-OMT and deoxyallosyl-DMT were produced from 5-O-mycaminosyl tylonolide (OMT) and demycinosyl tylosin (DMT), respectively. In addition, expression of TDP-deoxyallose biosynthetic genes plus three different sets of methyltransferase genes tylE and tylF (tylE, tylF and tylE-tylF) in the
S. venezuelae YJ028 supplemented with OMT and DMT, led to the production of 2'''-or/and 3'''-methyldeoxylallosyl OMT and 2'''-or/and 3'''-methyldeoxylallosyl DMT, respectively. These results describe more precise mycinose biosynthetic pathway and biosynthetic routes
to tylosin of S. fradiae.

키워드

저자

• Eun Ae CHOI [ Department of Chemistry and Nano Science, Ewha Womans University. ]
• Sung Ryeol PARK [ Department of Chemistry and Nano Science, Ewha Womans University. ]
• Ah Reum HAN [ Interdisciplinary Program of Bioengineering, Seoul National University. ]
• So Ra LEE [ Department of Chemistry and Nano Science, Ewha Womans University. ]
• Mi Sun AHN [ Department of Chemistry and Nano Science, Ewha Womans University. ]
• Eunji KIM [ Department of Chemistry and Nano Science, Ewha Womans University. ]
• Yeo Joon YOON [ Department of Chemistry and Nano Science, Ewha Womans University. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576