Jung-Hun KIM, Sang-Hwal YOON, Chong-Long WANG, Asad Ali SHAH, Hee-Jeong JANG, Jong-Wook SONG, Seo-Hee KANG, Hee-Kyoung RYU, Eui-Sung CHOI, Seon-Won KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A115095
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원문정보
초록
영어
Isoprene is a volatile C5 terpenoid that is released mainly from the leaves of many deciduous broad-leaved trees and some bacteria. Isoprene is present under standard conditions as a colorless liquid and it is the monomer of natural rubber and is a precursor to an immense variety of other naturally occurring compounds. Isoprene is an important chemical feedstock used in the synthetic rubber industry. About 800,000 tons per year of cis-polyisoprene are produced from the polymerization of isoprene, most of this polyisoprene is used in the tire and rubber industry. Isoprene is made from dimethylallyl diphosphate (DMAPP) by isoprene synthase via methyl erythritol 1-phosphate pathway (MEP). In this study, we constructed plasmid containing isoprene synthase (ispS) from three kinds of plant, Poplar alba, Poplar trichocarpa,and kudzu and introduced into E. coli to produce isoprene in E. coli. We confirmed isoprene production in E. coli and expecting that isoprene can be made in E. coli much more than now by our advanced metabolic engineering system. Isoprene formation is observed in the recombinant E. coli, which suggests E. coli as potential host strain for isoprene production. This work was supported by the EB-NCRC (Grant No. R15-2003-012-02001-0), KRIBB Research Initiative Program, and BK21 program of Korea.
키워드
IsopreneEscherichia coliisoprene synthase
저자
Jung-Hun KIM [ Division of Applied Life Science, Gyeongsang National University ]
Sang-Hwal YOON [ Division of Applied Life Science, Gyeongsang National University ]
Chong-Long WANG [ Division of Applied Life Science, Gyeongsang National University ]
Asad Ali SHAH [ Division of Applied Life Science, Gyeongsang National University ]
Hee-Jeong JANG [ Division of Applied Life Science, Gyeongsang National University ]
Jong-Wook SONG [ Division of Applied Life Science, Gyeongsang National University ]
Seo-Hee KANG [ Division of Applied Life Science, Gyeongsang National University ]
Hee-Kyoung RYU [ Korea Research Institute of Bioscience & Biotechnology ]
Eui-Sung CHOI [ Korea Research Institute of Bioscience & Biotechnology ]
Seon-Won KIM [ Korea Research Institute of Bioscience & Biotechnology ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동