Mycothiol that is comprised of N-acetylcysteine amide-linked to GlcN- α(1-1)-Ins has been identified as a major thiol in a number of actinomycetes. It plays to be its resistance to autoxidation and a significant role in the detoxification of thiol-reactive substances, such as formaldehyde, various electrophiles and antibiotics. Homology searches from the genome of Streptomyces peucetius ATCC 27952 have found a complete set of biosynthetic genes putatively involved in the biosynthesis of mycothiol. The four genes were designated as mshA, mshB, mshC and mshD. The four genes were colned into pIBR25 to make pMSH25. It is for expression of MSH biosynthetic genes under ermE* promoter. The pMSH25 has been transformed into Streptomyces venezuelae wild type, Streptomyces venezuelae YJ028, Streptomyces lividans TK24 and Streptomyces peucetius DM07. The mycothiol is being extracted from these Streptomyces transformed with recombinant plasmid and analyzing.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
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2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
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