Earticle

초록

영어

Putrescine (also known as 1,4-diaminobutane), is an important platform chemical with a wide range of applications in chemical industry. In particular, putrescine is currently polycondensed with adipic acid to synthesize nylon-4,6 (Stanyl®, DSM), a superior engineering plastic because of its high melting point and mechanical strength as well as excellent solvent resistance. Current
production of putrescine on industrial scale relies mainly on chemical synthesis from petrochemicals under environmentally harsh conditions. The chemical synthesis route is thus undesirable from an environmental point of view and human health standpoint. Therefore, there has been increasing need for biotechnological production of putrescine from renewable feedstock.Here we report a sustainable bio-based process for putrescine production using metabolically engineered strain of Escherichia coli. First, a base strain was constructed by inactivating the putrescine degradation and utilization pathways, and deleting the ornithine carbamoyltransferase chain I gene argI to make more precursors available for putrescine synthesis. Next, ornithine decarboxylase, which converts ornithine to putrescine, was amplified by a combination of plasmid-based and chromosome-based overexpression of the coding genes. Furthermore, the ornithine biosynthetic genes (argC-E) were overexpressed from the trc promoter, which replaced the native promoter in the genome, to increase the ornithine pool. Finally, strain performance was further improved by the deletion of the stress responsive RNA polymerase sigma factor RpoS, a well-known global transcription regulator that controls the expression of ca. 10% of the E. coli genes. The final engineered E. coli strain was able to produce 1.68 g L-1 of putrescine with a yield of 0.168 g per g glucose. Furthermore, high cell density cultivation allowed production of 24.2 g L-1 of putrescine with a productivity of 0.75 g L-1 h-1. The strategy reported here should be useful for the bio-based production of putrescine from renewable resources, and also for the development of strains capable of producing other diamines, which are important as nitrogen-containing platform chemicals. [This work was supported by the Korean Systems Biology Research Project of the Ministry of Education, cience and Technology through Korea Science and Engineering Foundation (M10309020000-03B5002-00000). Further supports by LG Chem Chair Professorship, Microsoft, World Class University Program of the Ministry of Education, Science and Technology, and KAIST Institute for the BioCentury are appreciated].

키워드

저자

• Ryeojin LEE [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST ]
• Zhi-Gang QIAN [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST ]
• Xiao-Xia XIA [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST ]
• Sang Yup LEE [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576