Earticle

초록

영어

Escherichia coli B and K12 derivatives are commonly used in laboratory and bioindustry due
to their fast growing and easy cultivation. After sequencing the complete genome of parent B
and K12 strains, we have the demanding task of investigating the proteomes of these strains
and obtaining valuable physiological information through comparative proteomics. Here, using two-dimensional gel electrophoresis coupled with MS/MS, we profiled the proteomes of an E. coli B derivative and three K12 derivatives grown with a defined medium at exponential phase and stationary phase, respectively. Over 100 proteins were differentially expressed, including those involved in cellular processes and stress responses, and those in the main metabolic pathways (glycolysis, TCA cycle, gluconeogenesis and fermentative pathway) and the synthesis pathways (amino acid biosynthesis, fatty acid biosynthesis, nucleotide synthesis
and energy synthesis). A correlation between the proteome profiling and metabolic pathway
analysis revealed significant metabolic differences among the four strains, which provided
valuable information for the production of various primary metabolites. Also, analysis of
amino acid synthesis and protein processing shed light on rational host selection for
recombinant protein production. In conclusion, comparative proteomic analysis revealed
systematic metabolic diversity among the four strains studied, and provided valuable
information for rational host strain. [This work was supported by the Korean Systems Biology
Project of the Ministry of Education, Science and Technology. Further supports by the LG
Chem Chair Professorship and KOSEF through the CUPS are appreciated].

키워드

저자

• Ryeojin LEE [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program) and BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST ]
• Xiao-Xia XIA [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program) and BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST ]
• Sang Yup LEE [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program) and BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576