The sphingolipid, representatively ceramides, and sphingosine, recently is being researched as signal transfer materials and induction apoptosis in cell and studied about cell-to-cell signaling including acceptor on the cell membrane representatively sphingosine 1-phosphae and sphingosylphosphorylcholine [1, 2]. And ceramides has been known that have roles of intermediate material of sphingolipids metabolism and importantly participate including apoptosis, cell cycle arrest, senescence, differentiation, and stress responses [1, 2]. The goals of this study are comparison of production of ceramides between Saccharomyces cerevisiae and transformants containing genes on sphingolipid pathway. The vector system was constructed with YCplac22 and YCplac33 that are under the control of lac promoter and include TRP1 and URA3 selectable marker, respectively. Ceramide were prepared by solvent extraction and quantitative analysis was analyzed by HPLC system with ELSD [3].
키워드
Saccharomyces cerevisiaeceramideYCplac
저자
Yong Ho NOH [ Dept. of Biological Engineering, Inha University ]
Se Kyung KIM [ Dept. of Biological Engineering, Inha University ]
Hyun Shik YUN [ Dept. of Biological Engineering, Inha University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
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