Earticle

초록

영어

A large number of natural and unnatural products from microorganisms such as biologically active compounds and commodity chemicals have attracted huge attention in the various industries including pharmaceutical and chemical industries. However, functional improvement of microorganisms meeting with commercial need is limited by the biological constraints developed during natural evolutionary process. “Metabolic Engineering” aims the purposeful redesign of the biological systems and requires the accurate information of the cellular metabolic networks and
proper tools for the reconstruction of the biological systems such as the regulatory elements and engineered proteins. The goal of “Synthetic Biology” is to synthesize whole biological system or its subsystem intentionally and designing parts such as the regulatory elements and functional gene should be necessarily predictable. Numerous regulatory elements such as promoter libraries and tunable intergenic regions (TIGRs) can be applied for the modulation of gene expression. However, without considering 5’-unstranslated region (5’-UTR) sequence, it is insufficient to precisely design and modulate the gene expression level. To address this issue,
in this study, we obtained 5’-UTR variants for gene expression in Escherichia coli, followed by GFP as a reporting system. Even if using same promoter sequence, 5’-UTR variants showed expression levels in a range of 100-fold. This indicates that additional information besides promoter strength, such as 5’-UTR, should be considered for precise gene expression.

키워드

저자

• Sang Woo SEO [ Department of Chemical Engineering, Pohang University of Science and Technology ]
• Gyoo Yeol JUNG [ Department of Chemical Engineering and School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576