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One-Step Production of Lactate-Based Polyesters Using Propionate CoA-Transferase and Polyhydroxyalkanoate Synthase

첫 페이지 보기
  • 발행기관
    한국생물공학회 바로가기
  • 간행물
    한국생물공학회 학술대회 바로가기
  • 통권
    2009 추계학술대회 및 국제심포지움 (2009.11)바로가기
  • 페이지
    pp.177-177
  • 저자
    Yu Kyung JUNG, Si Jae PARK, Sang Yup LEE
  • 언어
    한국어(KOR)
  • URL
    https://www.earticle.net/Article/A115078

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원문정보

초록

영어
Polylactic acid (PLA) has been considered as a good alternative to petroleum-based plastic. However, the current process for PLA synthesis is not simple: fermentative production of lactic acid followed by chemical polymerization. Differently from PLA which requires chemical polymerization of lactic acid or lactide, polyhydroxyalkanoates (PHAs) are synthesized in vivo by PHA synthase which polymerizes various (D)-hydroxyacyl-CoAs generated through diverse metabolic pathways in the cell1. In this study, we were able to show that poly(3-hydroxybutyrate-co-lactate) could be synthesized in recombinant Cupriavidus necator H16 (formerly, Ralstonia eutropha H16) expressing the Clostridium propionicum propionate CoA-transferase gene2, which allows generation of (D)-lactyl-CoA in the cell3. Also, four representative PHA synthases (type I-IV) were examined for their ability to synthesize lactate-containing polymers in Escherichia coli. This work was supported by LG Chem and by the
Korean Systems Biology Research Project (20090065571) of the Ministry of Education, Science and Technology. Further supports by the LG Chem Chair Professorship, Microsoft, and IBM SUR, WCU (World Class University) program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (R32-2008-000-10142-0) program are appreciated.

키워드

lactate-based polyesters one-step production propionate CoA-transferase PHA synthase

저자

  • Yu Kyung JUNG [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST ]
  • Si Jae PARK [ Corporate R&D, LG Chem Research Park, Daejeon, 305-380 / Present address: Green Chemistry Division, Chemical Biotechnology Research Center, Korea Research institute of Chemical Technology ]
  • Sang Yup LEE [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Department of Bio and Brain Engineering, Department of Biological Sciences, BioProcess Engineering Research Center, and Bioinformatics Research Center, KAIST ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
  • 설립연도
    1984
  • 분야
    공학>생물공학
  • 소개
    이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동

간행물

  • 간행물명
    한국생물공학회 학술대회
  • 간기
    반년간
  • 수록기간
    1985~2013
  • 십진분류
    KDC 476 DDC 576

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