Protein Engineering of Germacredinol Synthase (strep 13) from Streptomyces peucetius ATCC 27952: Characterization of N- and C-Terminal Region of Strep13
The Strep 13 encodes as sesquiterpene alcohol (germacredinol) synthaseencoding from Streptomyces peucetius ATCC27952. Complete open reading frame (ORF) of Strep 13 shows the production of germacredinol/geosmin. Here, we have explored the independent function of N-terminal and C-terminal protein of strep 13. Both terminal recombinant proteins were expressed at high level in Escherichia coli and analysis were carried out. N-terminal protein (~58.15 kDa) showed the Mg2+ dependent activity for the conversion of conversion of Farnesyl diphosphate to Germacrene D. In contrast, the expressed C-terminal domain of Strep13 has no farnesyl diphosphate cyclase activity.
Deepak UPRETI [ Department of Pharmaceutical, Institute of Biomolecule Reconstruction (iBR) ; Department of Biotechnology, Kathmandu University. ]
Gopal Prasad GHIMIRE [ Department of Pharmaceutical, Institute of Biomolecule Reconstruction (iBR) ; Department of Biotechnology, Kathmandu University. ]
Janardan LAMICHHANE [ Department of Pharmaceutical, Institute of Biomolecule Reconstruction (iBR) ; Department of Biotechnology, Kathmandu University. ]
Jae Kyung SOHNG [ Department of Pharmaceutical, Institute of Biomolecule Reconstruction (iBR) ; Department of Biotechnology, Kathmandu University. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
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