Soo Jung KIM, Ok-Hyeon YU, Jae Kweon PARK, Choul-Gyun LEE
언어
영어(ENG)
URL
https://www.earticle.net/Article/A115070
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원문정보
초록
영어
Ice binding proteins (IBPs) are diverse proteins with the capability of inhibiting ice crystal growth against in subzero environments for their survival. Some Antarctic algae are known to produce IBPs. In this study, two Antartic microalgae, named AnF0046 and AnF0048, were used for identifying IBPs. Extraction of genomic DNA and total RNA was conducted for confirmation of gene existence related to IBPs. After amplification of DNA by PCR with specially designed primers, single bands at 2500 bp and 1047 bp were found on the two agarose gels loaded DNA and synthesized cDNA from mRNA, respectively. When performing Blastn and Blastx for the homology search after sequencing, the sequences were similar to IBP isomer 1 of Chlamydomonas sp. CCMP681 with high identity. The amounts of IBP coding mRNA were expressed differentially under various period and/or temperature of cold shock treatments. It means that the amount of expressed IBPs were dependent upon cold shock period and cold shock temperature. This result suggests that AnF0046 and AnF0048 seems to have the putative IBP coding genes, whose sequeces have high identity with one of the known IBP isomer and expression of the genes were functions of cold shock temperature and period.
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동