Earticle

초록

영어

Gamma aminobutyric acid (GABA) is not only an essential inhibitory neurotransmitter which has a lot of physiological functions in humans but also a major monomer for a biobased and biodegradable polymer, polyamide 4. From an industrial point of view, the production of
GABA from the cheap source such as L-glutamate (L-MSG) is a completely reasonable and valuable process. The process can be well catalyzed by the enzyme glutamate decarboxylase (GAD) gained from various microorganism resources. In this work, we have conducted the α-decarboxylation of glutamate to yield GABA using pyridoxal phosphate-dependent GAD extracted from Escherichia coli. In order to obtain the intracellular enzyme, the strain was cultivated for 24
hours at 37 oC in LB medium and then sonicated, and the GAD was partially purified from the cell extract. The GAD showed a catalytic activity toward the conversion of L-MSG into GABA. With this
glutamate decarboxylase from E. coli, effects of reaction conditions such as temperature, pH, and substrate concentration were examined.

키워드

저자

• Thu Huong DINH [ Dept. of Chemical and Biochemical Engineering, Dongguk University ]
• Taek Jin KANG [ Dept. of Chemical and Biochemical Engineering, Dongguk University ]
• Keehoon WON [ Dept. of Chemical and Biochemical Engineering, Dongguk University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576