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Comparative Study of Enzyme Thermostability using Torsion Potentials in the Region of β-Turn Hong Seung Yeon and Young Je YooSchool of Chemical and Biological Engineering, Seoul
National University, Seoul 151-744, Korea Thermostable enzyme has been a key research topic
because it industrially gives a number of beneficial advantages. Various strategies to enhance
thermostability of enzymes have been proposed, however, an integrated strategy has not yet
reported. In this study, torsion potentials in the region of β-turn, as one of strategy to explain
enzyme thermostability, were analyzed between thermophilic and mesophilic enzymes. β-turn is
an element of secondary structure which consists of four residues and plays an important role in
protein structure directing folding pathways. The hypothesis of this study is that it might act to
enhance thermostability of enzymes by introducing favorable torsion angle in the region of β-turn
because β-turn is passively formed by other secondary structures. Thus, it may be assumed that
torsion potential of β-turns in mesophilic enzyme is bigger than thermophilic one. To demonstrate
this hypothesis, thirty sets of thermophlic and mesophilic enzyme pair were chosen through
several qualified criteria and intrinsic torsion potential equation was used. The statistical analysis
showed that torsion potential of mesophilic enzymes in the region of β-Turn is bigger than
thermophilic one at less than 5% level of significance. From this statistical result, optimizing
torsion angle in the region of β-turn can be used as a strategy for enhancing thermostability of
enzymes. ReferencesVijaya Parthiban, M. Michael Gromiha, Christian Hoppe, and Dietmar
Schomburg. PROTEINS:Structure, Function, and Bioinformatics 66:41-52(2007)Young Kee Kang, Kyoung Tai No, and Harold A. Scheraga. J. Phys. Chem 1996, 100, 15588-15598Saul R. Trevino, Stephanie Schaefer, J. Martin Scholtz, and C. Nick Pace. J Mol Biol. 2007 October 12; 373(1): 211-218

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저자

• Hong Seung YEON [ Dept. of Chemical and Biological Engineering, Seoul National University ]
• Young Je YOO [ Dept. of Chemical and Biological Engineering, Seoul National University, ]

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표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576