Earticle

초록

영어

Efficient immobilization of glucose oxidase (GOx) has been explored for many biological applications. In this work, we describe the constitutive expression of R5 tagged GOx in Pichia pastoris X-33 for biosilicification reaction. The gene encoding the GOx from Aspergillus niger KTCT 6278 was cloned into the pGAPZαC including saccharomyces cerevisiae α-factor secretion signal. Several transformants were selected on YPD medium with zeocin and the one showed excellent enzyme activity. Expressed recombinant proteins in the medium were concentrated and analyzed as a single band on SDS-PAGE. Influence of carbon sources, both glucose and glycerol was observed with growth rate, pH and enzyme activity. It is available high expression (1.96 U/ml) when glucose was consumed in a day. Although glycerol based medium
expended in four days was obtained high cell density but was expressed low (0.41 U/ml). Production of GOx through fermentation using glucose was optimized in a 1L reactor with controlling pH, DO, and aeration rate

키워드

저자

• Ji-hye AN [ Korea Institute of Science and Technology ]
• Yong-Hwan KIM [ Dept. of Chemical engineering, Kwangwoon University ]
• Youngsoon UM [ Korea Institute of Science and Technology ]
• Byoung-In SANG [ Korea Institute of Science and Technology, ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576