Spring model for enzyme deformation is proposed for the calculation of residual force as a new mechanical approach. This new modeling method assumes that enzyme moves like elastic body doing substrate docking and product release. It was validated by selection of forced and twisted region in cases of HIV-1 protease and bacteriophage T4 lysozyme. Enzyme flexibility has relation to enzyme activity because enzyme does flexible motion for catalysis. The selected regions using spring model were compared with activity-enhanced mutation regions in bacteriophage T4 lysozyme and Candida antarctica lipase B. It was found that the mutations at the helix edges located far away from twisted region increased enzyme activity in T4 lysozyme. In case of Candida antarctica lipase B, mutations at the most twisted region make enzyme activity increase. It will be discussed about relationship between enzyme flexibility and activity based on these data.
키워드
spring modelenzyme flexibilitybacteriophage T4 lysozymeCandida antarctica lipase BHIV-1 protease
저자
So Yeon HONG [ Graduate Program in Bioengineering, Seoul National University ]
Young Je YOO [ Graduate Program in Bioengineering, Seoul National University, ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동