Kommalapati Ramesh, Mouli Kalla Chandra, Tartte Vijaya
언어
한국어(KOR)
URL
https://www.earticle.net/Article/A114944
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원문정보
목차
An efficient in vitro plant regeneration system was developed through shoot proliferation from axillary buds of Tectona grandis (L.f), APNBV-1 (Andhra Pradesh North Badrachalam Venkatapuram-1) clone. Multiple shoots of high quality were produced in vitro from axillary bud explants. An average of 4.39 shoots/explant were obtained on Murashige and Skoog’s (MS) medium supplemented with plant growth regulators (PGRs) benzyl amino purine (BA), kinetin (KN), indole acetic acid (IAA), gibberillic acid (GA3), growth adjuvants casein hydrolysate (CH), adenine sulphate (Ads) and antioxidants ascorbic acid, polyvinyl pyrrollidine (PVP). Eighty five percent of rooting was observed in ex vitro rooting media containing IBA and vermiculite. In ex vitro rooting, single shoots with 2 to 3 nodes were subjected to IBA of different concentrations at different periods of time intervals. Direct rooting in vermiculite at 500 ppm concentration of IBA resulted in 4.3 number of roots with 2 cm length. Minimum response of rooting and length of roots were recorded at 100 ppm concentration of IBA. Planlets were transferred to plastic bags for short acclimatization stage in green house where they survived at 95%.
키워드
In vitro micropropagationex vitro rootingTectona grandisAPNBV-1 clone
저자
Kommalapati Ramesh [ Department of Biotechnology, S.V.University.Tirupati-517 502, A.P. India ]
Mouli Kalla Chandra [ Mouli Kalla Chandra1, | Department of Biotechnology, S.V.University.Tirupati-517 502, ]
Tartte Vijaya [ Department of Biotechnology, S.V.University.Tirupati-517 502, A.P. India ]
Corresponding author