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In vitro Plant Regeneration from Apical Bud and Nodal Segments of Anthocepahalus Cadamba – An important sacred and medicinal tree

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    Journal of Forest and Environmental Science 바로가기
  • 통권
    제25권 제2호 (2009.08)바로가기
  • 페이지
    pp.111-118
  • 저자
    M Kavitha, Kalaimagal, S Mercy, N Sangeetha, D Ganesh
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A114943

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원문정보

초록

영어
Multiple shoot induction and plant regeneration using apical bud and nodal explants of 100 year old tree of Anthocephalus cadamba, an important sacred and medicinal tree in India was achieved for the first time. Aseptic explants cultured in Murashige and Skoog (MS) medium augmented with different concentrations of BAP (0.1, 0.5, 1.0, 2.5, 5.0 and 10 mg/l), when maintained for 60 days, healthy shoots were induced in presence of BAP (1 mg/l). Lower concentrations of BAP (0.1 - 0.5 mg/l) induced only one shoot per explant. Increase in number of shoots per explant was observed in presence of higher concentrations of BAP (2.5, 5.0 and 10 mg/l). However, elongation of shoots was completely inhibited. Bud break and shoot regeneration was largely associated with seasonal factors. Apical buds cultured during June to August exhibited early bud break within two weeks of initial culture. In rest of the months, bud break and shoot regeneration was very slow irrespective of the various concentrations of BAP used in the medium. Explants sourced from three different maturity levels of shoots indicated that actively growing shoots from the mother plant with 1 – 2 nodal segments was more suitable for culture initiation than the explants collected from mature shoots at dormant stage. Regenerated shoots with 2 – 3 pairs of leaves when transferred to half strength MS medium fortified with IBA (1 mg/l), 60% of the shoots induced healthy roots, indicating the possibility of large scale micropropagation.

목차

ABSTRACT
 INTRODUCTION
 Materials and Methods
 Results and Discussion
 Acknowledgement
 References

키워드

Anthocephalus cadamba Shoot proliferation Micropropagation Plant regeneration Conservation

저자

  • M Kavitha [ Division of Plant genetic Improvement, Department of Biotechnology, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University ]
  • Kalaimagal, S Mercy [ Division of Plant genetic Improvement, Department of Biotechnology, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University ]
  • N Sangeetha [ Division of Plant genetic Improvement, Department of Biotechnology, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University ]
  • D Ganesh [ Division of Plant genetic Improvement, Department of Biotechnology, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    Journal of Forest and Environmental Science [산림과학연구]
  • 간기
    계간
  • pISSN
    2288-9744
  • eISSN
    2288-9752
  • 수록기간
    1981~2025
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 526 DDC 634

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