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Genetic Variability Based on Randomly Amplified Polymorphic DNA in Kacip Fatimah (Labisia pumila Benth & Hook f) collected from Melaka and Negeri Sembilan States of Malaysia

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    Journal of Forest and Environmental Science 바로가기
  • 통권
    제25권 제2호 (2009.08)바로가기
  • 페이지
    pp.93-100
  • 저자
    Subhash J Bhore, A H Nurul, Farida H Shah
  • 언어
    한국어(KOR)
  • URL
    https://www.earticle.net/Article/A114941

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원문정보

초록

영어
In Malaysia, Labisia pumila Benth & Hook f, popularly known as ‘Kacip Fatimah’ has been used traditionally to treat various elements of the woman’s health in Malay community. The objective of this study was to develop randomly amplified polymorphic DNA (RAPD) based DNA markers for the identification of L. pumila and to distinguish its three varieties from each other. Total DNA from nine accessions of L. pumila was extracted by CTAB method and polymerase chain reactions (PCR) were carried out to amplify the segments of DNA using different primers to develop DNA barcode using RAPD technique. To find out variety-specific DNA marker/s, twenty different 10-mer primer sequences with annealing temperature from 36-40 °C were evaluated in triplicate. Out of 20 random primers, two primers (OPA-1 and OPA-2/A10) were selected which produced reliable RAPD band patterns. To have DNA based handle, two RAPD amplification products were cloned and sequenced to determine the identity of the DNA. RAPD analysis using two random primers generated 72 discrete bands ranging in size 200 bp-3,000 bp. Fifty nine of these were polymorphic loci (82%) and thirteen were non-polymorphic loci (18%). A total of 32 bands polymorphic loci (72%) were amplified with primer OPA-1 and analyzed by cluster analysis and UPGMA (Unweighted Pair Group Method with Arithmetic) to present a dendogram depicting the degree of genetic relationship among nine accessions of L. pumila. Our results shows the reasonable genetic diversity among the L. pumila varieties and within varieties; and two RAPD marker sequences obtained could be used to identify L. pumila at species level.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHOD
 RESULTS AND DISCUSSION
 CONCLUSIONS
 ACKNOWLEDGEMENTS
 LIETERATURE CITED

키워드

Biodiversity DNA polymorphism Labisia pothoina Medicinal plants RAPD

저자

  • Subhash J Bhore [ Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong-Semeling Road, Bedong, 08100,Kedah Darul Aman, Malaysia ]
  • A H Nurul [ Molecular Biology Division, Melaka Institute of Biotechnology, Lot 7, Melaka International Trade Center City, Ayer Keroh, 75450, Melaka, Malaysia ]
  • Farida H Shah [ Novel Plants Sdn. Bhd., Taman Rambai Harmoni, Off Jalan Tanjuag Minyak, 75260, Melaka, Malaysia ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    Journal of Forest and Environmental Science [산림과학연구]
  • 간기
    계간
  • pISSN
    2288-9744
  • eISSN
    2288-9752
  • 수록기간
    1981~2025
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 526 DDC 634

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