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Effect of Extrinsic Factors on Differentiated Cardiomyocyte-like Cells from Human Embryonic Stem Cells

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 33 No 4 (2009.12)바로가기
  • 페이지
    pp.263-271
  • 저자
    Chang-Hyun Gil, Jae-Woo Jang, Won-Young Lee, Ze-Won Park, Jae-Ho Lee, Sun-Hwa Chung, Jung-Il Chae, Hyung-Min Chung
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A114898

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초록

영어
Cardiovascular diseases (CVDs) are one of the most cause of death around the world and fields of interest for cardiac stem cells. Also, current use of terminally differentiated adult cardiomyocytes for CVDs has limited regenerative capacity therefore any significant cell loss may result in the development of progressive heart failure. Human embryonic stem cells (hESCs) derived from blastocyst‐stage embryos spontaneously have ability to differentiate via embryo‐like aggregates (endoderm, ectoderm and mesoderm) in vitro into various cell types including cardiomyocyte. However, most effective molecule or optimized condition which can induce cardiac differentiation of hESCs is rarely studied. In this study, we developed both spontaneous and inductive cardiomyocyte‐like cells differentiation from hESCs by treatment of induced‐factors, 5‐azacytidine, BMP‐4 and cardiogenol C. On the one hand, spontaneous and inductive cardiomyocyte‐like cells showed that cardiac markers are expressed for further analysis by RT‐PCR and immunocytochemistry. Interestingly, BMP‐4 greatly improved mogeneous population of the cardiomyocyte‐like cells from hESCs CHA15 and H09. In conclusion, we verified that spontaneously differentiated cells showed cardiac specific markers which characterize cardiac cells, treated extrinsic factors can manage cellular signals and found that hESCs can undergo differentiation into cardiomyocytes better than spontaneous group. This finding offers an insight into the inductive factor of differentiated cardiomyocytes and provides some helpful information that may offer the potential of cardiomyocytes derived from hESCs using extrinsic factors.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

hESC Cardiomyocyte Differentiation BMP‐4

저자

  • Chang-Hyun Gil [ Graduate School of Life Science, CHA Stem Cell Institute, College of Medicine, CHA University ]
  • Jae-Woo Jang [ Graduate School of Life Science, CHA Stem Cell Institute, College of Medicine, CHA University ]
  • Won-Young Lee [ CHA Bio & Diostech Co., Ltd. ]
  • Ze-Won Park [ Graduate School of Life Science, CHA Stem Cell Institute, College of Medicine, CHA University ]
  • Jae-Ho Lee [ CHA Bio & Diostech Co., Ltd. ]
  • Sun-Hwa Chung [ Graduate School of Life Science, CHA Stem Cell Institute, College of Medicine, CHA University ]
  • Jung-Il Chae [ Graduate School of Life Science, CHA Stem Cell Institute, College of Medicine, CHA University ]
  • Hyung-Min Chung [ Graduate School of Life Science, CHA Stem Cell Institute, College of Medicine, CHA University, CHA Bio & Diostech Co., Ltd. ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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