Earticle

초록

영어

To examine the differential expression of proteins during the cycling (70～80% confluences) and G0/G1 (full confluences) phases in porcine fetal fibroblast cells, we used a global proteomics approach by 2‐D gel electrophoresis (2‐DE) and MALDI‐TOF‐MS. Cycling cell were harvested at approximately 70% to 80% confluent state while cells in G0/G1 phase were recovered after maintenance of a confluent state for 48 hr. Cellular proteins with isoelectric points ranging between 3.0～10.0, were analyzed by 2‐DE with 2 replicates of each sample. A total of approximately 700 spots were detected by 2‐D gels stained with Coomassie brilliant blue. On comparing the cell samples obtained from the cycling and G0/G1 phases, a total of 13 spots were identified as differentially expressed proteins, of which 8 spots were up‐regulated in the cycling cell and 5 were up‐regulated in the G0/G1 phase. Differentially expressed proteins included K3 keratin, similar to serine protease 23 precursor, protein disulfide‐isomerase A3, microsomal protease ER‐60, alpha‐actinin‐2, and heat‐shock protein 90 beta. The identified proteins were grouped on the basis of their basic functions such as molecular binding, catabolic, cell growth, and transcription regulatory proteins. Our results show expression profiles of key proteins in porcine fetal fibroblast cells during different cell cycle status.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

저자

• Rong Xun Han [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
• Hong-Rye Kim [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
• Yunfei Diao [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
• Myung Youn Kim [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
• Chang Sik Park [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University ]
• Dong Il Jin [ Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University ] Corresponding author

참고문헌

발행기관

간행물

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• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636