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Expression of Placenta-Related Genes (Cdx2 and GATA6) in Cloned Porcine Development

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 33 No 4 (2009.12)바로가기
  • 페이지
    pp.195-202
  • 저자
    Byung-Hyun Cha, Seongsoo Hwang, Hwi-Cheul Lee, Mi-Rung Park, Gi-Sun Im, Jae-Seok Woo, Soo-Bong Park, Jae-Hyeon Cho, Yeoung-Gyu Ko
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A114855

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원문정보

초록

영어
Abnormal development and fetal loss during the post‐implantation period are key concerns in the production of cloned animals by somatic cell nuclear transfer (SCNT). We hypothesized that the problems in cloned porcine offspring derived from SCNT are related to interactions between the conceptus and the endometrial environment. In the present study, we investigated expression patterns in the formation of placenta‐related genes (Cdx2 and GATA6) in whole in vivo normal porcine embryos (from single cell to blastocyst) and each tissue of a normal fetus at Days 25, 35 and 55 by quantitative mRNA expression analysis using real‐time PCR. The expression of
Cdx2 and GATA6 mRNA increased to around the blastocyst stage. These genes were gradually decreased from the peri‐implantation to post‐implantation stage. Moreover, we examined the expression patterns of Cdx2 and GATA6 in Day 35 normal and SCNT cloned fetuses by the same methods. And, the level of Cdx2 and GATA6 gene expression in the extraembryonic tissue of SCNT was significantly higher than that of control tissues. From the present results, it can be postulated that the aberrant expression of Cdx2 and GATA6 genes in the endometrial and extraembryonic tissues at pre‐ and peri‐implantation stages may be closely related to the lower fficiency of animal cloning.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Cdx2 GATA6 Pre‐ and peri‐implantation Gene expression)

저자

  • Byung-Hyun Cha [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Seongsoo Hwang [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Hwi-Cheul Lee [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Mi-Rung Park [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Gi-Sun Im [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Jae-Seok Woo [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Soo-Bong Park [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ]
  • Jae-Hyeon Cho [ Gyeongsang National University, Jinju 660-701, Korea ]
  • Yeoung-Gyu Ko [ Division of Animal Biotechnology, National Institute of Animal Science, RDA ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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