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In Vitro Growth of Bovine Preantral Follicle under Different Culture Conditions

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 33 No 4 (2009.12)바로가기
  • 페이지
    pp.189-194
  • 저자
    Hyun-Joo Lim, Dong-Hoon Kim, Gi-Sun Im, Seongsoo Hwang, Kwang-Soo Baek, Byeong-Soon Jeon, Sung-Jai Park, Hyeon-Shup Kim, Jeong-Mook Lim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A114854

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원문정보

초록

영어
The objective of this study was to determine effects of different culture media. Preantral follicles were mechanically extracted from bovine ovaries and cultured for 16 days in tissue culture medium (TCM)‐199, DMEM or alphaminimal essential medium (α‐MEM) + 10% FBS + 0.1 mg/ml sodium pyruvate + 100 mIU/ml FSH. The collected primary follicles from ovary were higher than the primary and secondary follicles. The survival rates of the follicles in TCM‐199 were significantly higher (p<0.05) than those in DMEM and α‐MEM. The diameter of the follicles progressively increased during 12 days of culture. The maximum size (139.1±5.4 μm) reached on Day 12 of the in vitro culture and decreased on Day 16. These results suggest that in a culture of bovine preantral follicles, TCM‐199 is an optimal medium and a longer‐term culture of preantral follicles (>12 days) may be needed to form antra.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Medium Bovine Preantral follicle TCM‐199)

저자

  • Hyun-Joo Lim [ Dairy Science Division, National Institute of Animal Science, RDA ] Corresponding author
  • Dong-Hoon Kim [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Gi-Sun Im [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Seongsoo Hwang [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Kwang-Soo Baek [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Byeong-Soon Jeon [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Sung-Jai Park [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Hyeon-Shup Kim [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Jeong-Mook Lim [ Research Institute for Agriculture and Life Sciences, Department of Animal and Food Biotechnology,Seoul National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 33 No 4

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