Yang-Ho JO, Dong-Min YOO, Bo-Youn JANG, Yong-Il HWANG, Sun-Uk CHOI
언어
영어(ENG)
URL
https://www.earticle.net/Article/A114632
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
Guanosine tetraphosphate (ppGpp) responding to carbon and energy starvation in bacteria acts as positive regulator for antibiotic production in some Streptomyces. The relA gene of Streptomyces natalensis producing a natamycin, which is widely used in food industry to prevent mold contamination of cheese and other non-sterile foods, was searched with PCR using the primers designed from the two highly conserved regions of RelA in Streptomyces species. In a previous study, 832-bp product was gained by PCR and had a high similarity to the expected region of a relA gene. An intact 2.6-kb relA gene of S. natalensis was obtained by Southern blot as well as Colony PCR using the 832-bp PCR product as probe. The obtained relA was inserted into pSET152ET, over-expression vector, and then introduced into Streptomyces strains. Also, a relA expression was assayed in the exconjugant and its phenotype change was compared with the control strain containing only pSET152ET vector. [This work was supported by the Korea Science and Engineering Foundation(KOSEF) grant funded by the Korean government(MEST) (No. 2009-0058974)]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동