Earticle

초록

영어

Nattokinase is a potent fibrinolytic enzyme that is considered to be a promising agent for thrombosis therapy. The enzymes were discovered from various sources, such as Japanese natto, Korean Chungkookjang soy sauce, and Chinese douchi. Based on its food origin and relatively strong fibrinolytic activity, nattokinase has advantages over other commercially used medicine in preventative and prolonged effects, convenient oral administration, and stability in the gastrointestinal tract.In this study, Bacillus subtilis was cultivated in flask and fermentor to produce nattokinase. Glucose and NH4Cl were used as carbon and nitrogen sources, respectively, with peptone, yeast extract, or tryptone supplementation. Glucose concentration was fixed at 10 g/L. New method of assaying nattokinase activity was suggested. In flask culture, the highest cell growth (optical density monitored at 600 nm) and nattokinase activity were obtained with 50 g/L of peptone supplementation. At this condition, nattokinase activity was 630 unit/ml. In batch culture of B. subtilis in fermentor, optical density increased to 10 at 6 h and then gradually decreased. Nattokinase activity also increased with cell growth, showing growth-associated production. With 50 g/L of peptone supplementation, the highest nattokinase activity of 3400 unit/ml was obtained at 10 h.

키워드

저자

• Young-Han CHO [ Department of Chemical Engineering, Chungbuk National University ]
• Beom Soo KIM [ Department of Chemical Engineering, Chungbuk National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576