A bacterial flavin-containing monooxygenase (FMO) gene was cloned from Methylophaga aminisulfidivorans MPT, and a plasmid pBlue 1.7 was constructed to express the bacterial fmo gene in E. coli. The recombinant E. coli harboring the pBlue 1.7 plasmid produced 95 mg/L of indirubin and 5 mg/L of indigo in tryptophan medium (2g tryptophan, 10g NaCl, 5g yeast extract per liter) containing several amino acids (cysteine 0.05%, aspratic acid 0.005% and arginine 0.005%, w/v). The aim of this study was the investigation of the optimum indirubin production conditions through batch culture. The optimum conditions were as follows: tryptophan concentration 1g/L, pH 7.0, air supply 1.0 vvm and culture temperature 35℃. Under these conditions, 100 mg/L indirubin and 4mg/L indigo were produced.
키워드
flavin containing monooxygenaseindirubinindigo
저자
Gui Hwan HAN [ Department of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, Gwangju 501-759. ]
Seong Eun BANG [ Department of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, Gwangju 501-759. ]
Si Wouk KIM [ Department of Environmental Engineering, BK21 Team for Biohydrogen Production, Chosun University, Gwangju 501-759. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
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