Earticle

초록

영어

Flavonoids are classified as Chalcone, Flavone, Flavanon and etc. Phloretin is a kind of chalcone compounds, and Apigenin and Chrysin are a flavone that are known for their
antitumor, antioxidant, antiarthritic, anti-amyloid and anti-inflammatory properties. However,
very poor oral bioavailability makes them largely ineffective in vivo. In previous studies, Omethylated
or hydroxylated flavonoid compounds are certificated more effective absorption in
vivo than flavonoid compounds. Twelve microorganisms were initially screened for their
abilities to catalyze biotransformation of chrysin and phloretin. Chrysin was orthohydroxylated
by Streptomyces ceolicolor to produce baicalein (5,6,7-tetrahydroxyflavone), which was confirmed using authentic baicalein with mass analysis. Maximum conversion was 12 %, which was achieved for 4 hours of reaction, and the substrate (chrysin) and reaction product (baicalein) was completely metabolized after 6 hours of reaction. Three kinds of Cytochrome P450 inhibitor, Coumarin, Erythromycin and Quinidine was added with 0.5mM final concentration. Coumarin had no effect on the bioconversion of chrysin and Quinidine inhibited conversion of chrysin into baicalein about 45 ~ 70 %, and Erythromycin completely blocked the bioconversion, which suggest bioconversion of chrysin was mediated through P450 system of Streptomyces Ceolicolor. Addition of different kinds of detergent (Triton X- 100, Tween 80, Brij 35) into reaction mixture, was not effective for the enhancement of bioconversion using Streptomyces Ceolicolor.In the biotransformation phloretin using Streptomyces avermitilis, two main products were identified in GC/MS analysis. They were interpreted as hydroxylated products of phloretin in A-ring at different position.

키워드

저자

• Woo-il KIM [ Department of Chemical Engineering, Dong-A University ]
• Jongki LEE [ Department of Chemical Engineering, Dong-A University ]
• Jiwon ROH [ Department of Chemical Engineering, Dong-A University ]
• Chang-Ju JEON [ Department of Chemical Engineering, Dong-A University ]
• KwonYoung CHOI [ School of Chemical and Biological Engineering, Seoul National University ]
• TaeJin KIM [ School of Chemical and Biological Engineering, Seoul National University ]
• Byung-Gee KIM [ School of Chemical and Biological Engineering, Seoul National University ]
• June-Hyung KIM [ Department of Chemical Engineering, Dong-A University ]

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발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576