Earticle

초록

영어

Microbial persistence is one of the mechanisms that exhibit tolerance to antibiotics. Unlike resistance, persistence has involves no genetic modification. In other words, persisters (survivors after antibiotics treatment) are not genetically different from the dead cells. Though
mechanism of persistence still remains as black box, it is well accepted that microbial persistence is the cause of secondary infection of pathogen. After antibiotics degradation, persister population grows again and makes second infection.Inhibition of persister formation is
believed to be useful in treating bacterial pathogenesis that is not well cured by antibiotics and will reduce the chance of resistant cell emergence. We screened persistence inhibitor using 6,800 chemical compounds library. Exponential phase cultures in 96well plate were exposed to chemical compound (5uM) and ampicillin at 50ug/ml concentration, which killed normal cells but did not kill persister (~0.1% of total cells). In first screening, we screened 52 chemical compound which killed bacteria almost completely. To exclude chemicals which do not act as persistence inhibitor but function as simple antibiotics, second screening was performed. Nine chemical
compounds were selected and indentified. Currently, we are investigating persistence mechanism using these chemicals.

키워드

저자

• Jun-Seob KIM [ School of Biotechnology and Bioengineering, Sungkyunkwan University ]
• Paul HEO [ School of Biotechnology and Bioengineering, Sungkyunkwan University ]
• Dae-Hyuk KWEON [ School of Biotechnology and Bioengineering, Sungkyunkwan University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576