Earticle

초록

영어

The bio-conversion of lignocellulosic biomass to ethanol has been extensively studied in bioenergy field. When lignocellulosic biomass converts to ethanol, enzyme takes lots of part of whole cost. Therefore, cellulase production is one of the important processes for the successful enzymatic conversion of cellulosic biomass to ethanol. Cellulase is multi-complex enzyme containing endo-glucanase, exoglucanase and ß-glucosidase (cellobiase). The production of cellulase has been mainly carried out by using Trichodema reesei. Also, suitable cellulase composition was important for the effective saccharification of lignocellulosic biomass and strain having high level production of cellulase should be developed for hydrolysis. In this study, T. reesei KCTC 6950, which was mutant derived from wild type QM 6a, was used as a starting strain for mutation by proton beam irradiation. The selection of cellulase over-producing mutants was performed on agar plate of Mandels mineral salts solution including carbon source such
as carboxymethylcellulose (CMC) and phosphoric acid-swollen cellulose. Positive mutants were selected on the basis of larger clear zone or the formation of very clear halo. Selected mutants were tested for the production of cellulase in liquid culture.

키워드

저자

• You Ree JUNG [ Dept. of Chemical & Biological Engineering, Korea University. ]
• Seong Woo KANG [ Dept. of Chemical & Biological Engineering, Korea University. ]
• Hyun Yong SHIN [ Dept. of Chemical & Biological Engineering, Korea University. ]
• Jong Ho LEE [ Dept. of Chemical & Biological Engineering, Korea University. ]
• Seung Wook KIM [ Dept. of Chemical & Biological Engineering, Korea University. ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576