Mi Ran CHOI, Ji Suk JANG, Zhong QION, Yeong Jin KIM, Sung-Koo KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A114546
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
영어
WSSV caused major viral diseases in shrimp aquaculture industry. Viral envelop proteins of WSSV play important roles on the virus infection. This study was carried out for the identification of mRNA transcriptional level at various WSSV infection stages using Reverse Transcription-PCR (RT-PCR).mRNAs of shrimps were isolated from shrimp organs at various infection stages after WSSV infection. Through the comparison of mRNA transcriptional levels of infected shrimp organs, it was confirmed that the vaccination of shrimp delayed viral gene transcription for 5~ 10 days. However, the general mRNA transcriptional pattern of vaccinated shrimps showed similar trend to that of unvaccinated shrimps. RT-PCR results from infected shrimps indicated that VP28 was not suitable for the analysis of infection stage due to early and high level transcription in every sample. But, VP26 was a useful marker of infection stage owing to infection stage related RT-PCR transcriptional pattern. RT-PCR of VP26 could provide information on the shrimp mortality and virus propagation.In further study, infection patterns will be analyzed using mRNA level via RTPCR from infected shrimps using other primer sets.
키워드
WSSVRT-PCRenvelop protein
저자
Mi Ran CHOI [ Dept. of Biotechnology, Pukyong National University ]
Ji Suk JANG [ Dept. of Biotechnology, Pukyong National University ]
Zhong QION [ Dept. of Biotechnology, Pukyong National University ]
Yeong Jin KIM [ Dept. of Biotechnology, Pukyong National University ]
Sung-Koo KIM [ Dept. of Biotechnology, Pukyong National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동