Although Saccharomyces cerevisiae is capable of fermenting galactose into ethanol, ethanol yield and productivity from galactose are significantly lower than those from glucose. An inverse metabolic engineering approach was undertaken to improve ethanol yield and productivity from galactose in S. cerevisiae. The characterization of genetic perturbations in the isolated transformants revealed three target genes whose overexpression elicited an enhanced galactose utilization. One confirmatory (SEC53) and two novel targets (SNR84 and a truncated form of TUP1) were identified as overexpression targets that potentially improve galactose fermentation. Moreover, the overexpression of tTUP1 significantly shortened lag periods that occurs when substrate is changed from glucose to galactose. The results presented in this study illustrate that alteration of global regulatory networks through overexpression of the identified targets (SNR84 and tTUP1) is as effective as overexpression of a rate limiting metabolic gene (PGM2) in the galactose assimilation pathway for efficient galactose fermentation in S. cerevisiae. In addition, these results will be industrially useful in the biofuels area as galactose is one of the abundant sugars in marine plant biomass such as red seaweed as well as cheese whey and molasses.
키워드
S.cerevisiaeGalactosetTUP1
저자
Ki-Sung LEE [ School of Biotechnology and Bioengineering, Sungkyunkwan University ]
Min-Eui HONG [ School of Biotechnology and Bioengineering, Sungkyunkwan University ]
Seok-Chae JUNG [ School of Biotechnology and Bioengineering, Sungkyunkwan University ]
Young-Je SUNG [ School of Biotechnology and Bioengineering, Sungkyunkwan University ]
Dae-Hyuk KWEON [ School of Biotechnology and Bioengineering, Sungkyunkwan University ]
Yong-Su JIN [ Department of Food Science and Human Nutrition, and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801. ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동