Earticle

초록

영어

Expressing anti-apoptotic cloned-gene inside cells is a widely used strategy for inhibiting apoptosis. In our previous study, we reported Bombyx mori 30Kc gene could improve the production of recombinant proteins in Chinese hamster ovary (CHO) cells. In this study we reengineered the CHO cells for the production of humanized monoclonal antibodies (mAb) directed against leukemia with 30Kc6 gene and 30Kc19 gene, respectively. Over-expression
of 30Kc6 in CHO cells increased the cell viability by 40.8% and mAb production by 2.31-fold after serum deprivation, but the cells over-expressing 30Kc19 varied a lot. In the case of 30Kc6 gene, the specific production rate of the cells expressing 30Kc6 was also improved. The 30Kc6 gene can increase the cell viability and productivity because it maintains the mitochondrial
membrane potential (MMP) and prevents the downstream cascade responses for apoptosis. Our results indicate that 30Kc6 outperforms 30Kc19 in cell death-protective capability and it is potent to improve the productivity of monoclonal antibody in CHO cells.

키워드

저자

• Zesong WANG [ School of Chemical and Biological Engineering, Seoul National University, 2The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China. ]
• Ju Hyun PARK [ School of Chemical and Biological Engineering, Seoul National University ]
• Hee Ho PARK [ School of Chemical and Biological Engineering, Seoul National University ]
• Wensong TAN [ The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China. ]
• Tai Hyun PARK [ School of Chemical and Biological Engineering, Seoul National University ]

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576