Seung Hun LEE, Jung Soo KIM, Sam Woong KIM, Sung-Koo KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A114539
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원문정보
초록
영어
E. tarda ghost vaccines may provide immune efficacy against edwardsiellosis in farmed fish. However, the leakage of antibioticresistance gene from cloned cells leads to occurrence of antibiotic resistant bacteria and negative effect on environment. Thus, plasmid based on an aspartate-semialdehyde-dehydrogenase (asd) gene was constructed for the E. tarda ghost vaccine to prevent the spread of the antibiotic resistance gene in environment. Target gene for the construction of auxotrophic E. tarda was asd gene, which is an essential gene for the construction of cell wall. The auxotrophic strain was generated by allelic exchange using a suicidal vector harboring DNA segments for asd gene deletion. A pBR origin, asd gene from pYA3342 and E lysis gene controlled by lambda PR/cI regulatory system from pGhost were transfered into the recombinant plasmid. Efficient promoter and signal peptide systems on the vector will be studied for better antigen expression and delivery of the antigen to outer membrane. The auxotrophic E.tarda ghost vaccine with antigen delivery system can lead to safe environment with as antibiotic-free system and increase the protection efficacy against dwardsiellosis.
키워드
E.tardaGhost vaccineedwardsiellosis
저자
Seung Hun LEE [ Dept. of Biotechnology, Pukyong National University ]
Jung Soo KIM [ Dept. of Biotechnology, Pukyong National University ]
Sam Woong KIM [ Dept. of Biotechnology, Pukyong National University ]
Sung-Koo KIM [ Dept. of Biotechnology, Pukyong National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동