The aim of this study was to investigate the microbial population dynamics in the salted Chinese cabbage and the recycled brine solution. For this, we performed the polymerase chain reactiondenaturing gradient gel electrophoresis (PCR-DGGE) analysis using a universal primer pair of 338f-GC and 518r, with the genomic DNAs of bacterial cells from salted Chinese cabbage and the brine solution after repeated salting processes in 13% salt solution for 15hr at 10℃. As results, the bacterial species identified were Pseudoalteromonas sp. Halomonas sp. Cobetia sp. Bacillus sp. and Lactobacillus sakei. Particularly, while Pseudoalteromonas sp. and Halomonas sp. were found in the brine solution, Lb. sakei was only detected in Chinese cabbage samples. Along with the repeated use of brine solution, the halotolerant bacteria were accumulated to become a dominant group in the brine solution and Lb. sakei disappeared in the salted Chinese cabbage. This result reveals that repeated use of brine solution to produce the salted Chinese cabbage result in unsafe and unfavorable effects on kimchi fermentation. From this experiment, PCR-DGGE analysis was proven useful for investigating microbial dynamics in foods.
키워드
salting processbrinechinese cabbage
저자
Jee Yun JUNG [ Dept.of Food Science and Technology, Chungbuk National University ]
Ki-Seon YOO [ Dept.of Food Science and Technology, Chungbuk National University ]
Ji Eun KIM [ Dept.of Food Science and Technology, Chungbuk National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동