Earticle

초록

영어

The analysis of microbial structure and dynamics in the methanogenesis, associated with performance pattern, were investigated in two anaerobic batch digesters using swine wastewater at the same process condition. All two bioreactors were initially seeded with anaerobic sludge originating from a local municipal wastewater treatment plant, based on 1% of TSS (total suspended solids), and processed in the batch mode for 80 days. The analysis of process performance of methanogenic digesters called M1, M2 revealed different process pattern in the same condition of performance. The accumulated amounts of methane production in the M1 and M2 were 7.9 L/L, and 4.5 L/L, respectively; in addition, the starting points of acetate reduction were 37th day, and 47th day; the initial points of propionate consumption were 51st day, 61st day, respectively. For the determination of causes to arouse differences between both digesters, denaturing gradient gel electrophoresis (DGGE) was carried out, and the populations of bacteria and archaea were monitored, at order levels with real-time PCR based on the 16S rRNA gene. The bacterial population in M1 was higher than that in M2 throughout batch process, and in case of Archaea, Methanobacteriales (MBT) and Methanomicrobiales (MMB) were predominated in both of digesters among archaea. The initially bacterial concentration in M1 was somewhat higher than in M2; in contrast, the archaeal concentration in M1 was a little lower than in M2. In case of M1, MBT and MMB showed the outstanding increase of their populations at the reduction points of acetate and propionate. However, in case of M2, the population increase of MBT was not so high at the reduction points of acetate and propionate; moreover, it was not observed for the population of MMB at the propionate reduction period to increase. For the correlation analysis with redundancy analysis (RDA), MMB was more deeply associated to acetate and propionate concentrations than MBT was. Based on these results, we concluded and that MMB increase in the propionate reduction stage must be important to the abundant methane production, even though both MBT and MBT were predominated in all digesters, and that the higher initial bacterial concentration was, the better methanogenesis of MMB, MBT was due to the increase of acidogenic activity of bacteria. Through this finding, it is possible to investigate why the uplicated trials of methano-process at the same condition lead to different results, which can be basically applied to the optimization of methanogenesis in the future. Acknowledgements This work was financially supported in part by the Korea Ministry of Environment (MOE) as Human Resource Development Project for Waste to Energy, Manpower Development Program for Energy & Resources by the Ministry of Knowledge and Economy (MKE), Korea Energy Management Corporation (2006-N-BI-02-P-09-0-000-2007) program and by the BK-21 program.

키워드

저자

• Woong KIM [ School of Environmental Science and Engineering, POSTECH ]
• Su Jeong OH [ School of Environmental Science and Engineering, POSTECH ]
• Huijuan LI [ School of Environmental Science and Engineering, POSTECH ]
• Duong NGUYEN [ School of Environmental Science and Engineering, POSTECH ]
• Biwen ZHOU [ School of Environmental Science and Engineering, POSTECH ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576