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학생구두발표 (한국어발표) : 좌장: 이상현 교수(신라대학교)

Metabolic Engineering of Escherichia coli for the Production of Polylactic Acid and its Copolymers

첫 페이지 보기
  • 발행기관
    한국생물공학회 바로가기
  • 간행물
    한국생물공학회 학술대회 바로가기
  • 통권
    2009 추계학술대회 및 국제심포지움 (2009.11)바로가기
  • 페이지
    pp.129-129
  • 저자
    Yu Kyung JUNG, Tae Yong KIM, Si Jae PARK, Sang Yup LEE
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A114519

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원문정보

초록

영어
Polylactic acid (PLA) is a promising biomass-derived polymer, but is currently synthesized by a two-step process: fermentative production of lactic acid followed by chemical polymerization. Here we report production of PLA homopolymer and its copolymer, poly(3-hydroxybutyrate-co-lactate), P(3HB-co-LA), by direct fermentation of metabolically engineered Escherichia coli. Introduction of the heterologous metabolic pathways involving engineered propionate CoA-transferase and polyhydroxyalkanoate (PHA) synthase for the efficient generation of lactyl-CoA and incorporation of lactyl-CoA into the polymer, respectively, allowed synthesis of PLA and P(3HB-co-LA) in E. coli, but at relatively low efficiency1. In this study, the metabolic pathways of E. coli were further engineered based on in silico genome-scale metabolic flux analysis in
addition to rational approach2. Using this engineered strain, PLA homopolymer and P(3HB-co-LA)
copolymers containing up to 70 mol% lactate could be produced up to 11 wt% and 46 wt% from glucose, respectively. Thus, the combined approaches of systems-level metabolic engineering and enzyme engineering allowed efficient bio-based one-step production of PLA and its opolymers. This work was supported by LG Chem and by the Korean Systems Biology Research Project (20090065571) of the Ministry of Education, Science and Technology. Further supports by the LG Chem Chair Professorship, Microsoft, and IBM SUR, WCU (World Class University) program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (R32-2008-000-10142-0) program are appreciated.

키워드

polylactic acid lactate-based copolymers systems metabolic engineering metabolic flux analysis

저자

  • Yu Kyung JUNG [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST ]
  • Tae Yong KIM [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST ]
  • Si Jae PARK [ Corporate R&D, LG Chem Research Park, Daejeon, 305-380 / Present address: Green Chemistry Division, Chemical Biotechnology Research Center, Korea Research institute of Chemical Technology ]
  • Sang Yup LEE [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Department of Bio and Brain Engineering, Department of Biological Sciences, BioProcess Engineering Research Center, and Bioinformatics Research Center, KAIST ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
  • 설립연도
    1984
  • 분야
    공학>생물공학
  • 소개
    이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동

간행물

  • 간행물명
    한국생물공학회 학술대회
  • 간기
    반년간
  • 수록기간
    1985~2013
  • 십진분류
    KDC 476 DDC 576

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