Seung Jun PARK, Sam Woong KIM, Sul Hee PARK, Sung-Koo KIM
언어
영어(ENG)
URL
https://www.earticle.net/Article/A114509
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원문정보
초록
영어
The bacterial ghost system is a novel vaccine delivery system. It provides versatile carrier functions for foreign antigens with excellent natural intrinsic adjuvant properties. The optimized medium condition for the ghost bacteria, vaccines E. coli K-12/pHCE-InaN-GAPDHGhost27SDM were determined as 10 g/L glucose, 5 g/L yeast extract and 50 mg/L thiamine with Riesenberg's semi-defined medium according to one-at-a-time method. The optimal batch fermentation parameters were determined as 10% inoculum size, 300 rpm agitation and 2.0 vvm aeration for the highest production of 3.7 g dcw/L. The nutrient feeding strategy with Riesenberg's defined medium for ghost bacteria vaccine fed-batch process was evaluated. Fed-batch fermentation for the optimized ghost bacteria vaccine production was carried out in 4 phases; batch, fed-batch, induction and heat-shock phases. The heat-shock phase was high temperature holding phase at 47℃ for 2 h to increase ghost cell efficiency. Under the optimal condition of fed-batch fermentation, the cell density of ghost bacteria reached 35 g dcw/L with 99.9% of ghost formation efficiency.
키워드
Ghost vaccinestreptococcosisfed-batch fermentationmass production
저자
Seung Jun PARK [ Dept. of Biotechnology, Pukyong National University ]
Sam Woong KIM [ Dept. of Biotechnology, Pukyong National University ]
Sul Hee PARK [ Dept. of Biotechnology, Pukyong National University ]
Sung-Koo KIM [ Dept. of Biotechnology, Pukyong National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동