Most of the biological mechanisms such as DNA replication, transcription, and gene regulation are based on molecular interactions between DNA and protein (or enzyme). It has been demanded to develop more functional probe molecules, which are useful and proper for analyzing molecule-level study between DNA and protein. In this study, we presented new approaches using DNA oligomer with unnatural nucleobases as novel DNA probes. In particular, nitric oxide (NO)- damaged nucleobases such as oxanine (Oxa) and hypoxanthine (Hyp) were explored in terms of their biotechnological applications. We prepared Oxa- or Hyp-containing DNA oligomers and employed as novel substrates for DNA-recognizing enzymes or DNA-binding proteins. These methods and the obtained results will be useful for elucidation of molecular studies based on DNA-protein interactions.
키워드
Biomoelcular interactionOxanineHypoxanthine
저자
Seung Pil PACK [ Dept. of Biotechnology and Bioinformatics, Korea University ]
Euikyong JANG [ Dept. of Biotechnology and Bioinformatics, Korea University ]
Chan-hee CHO [ Dept. of Biotechnology and Bioinformatics, Korea University ]
Keisuke MAKINO [ Institute of Advanced Energy, Kyoto University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동