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Biotron Bioreactor Symposium : Recent Advances and Application of Biomanufacturing Technology : 공동개최 : 바이오트론 : 좌장: 박진병 교수(이화여자대학교)
Enhancement of NDV Vaccine productivity
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- 발행기관
- 한국생물공학회 바로가기
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- 간행물
- 한국생물공학회 학술대회 바로가기
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- 통권
- 2009 추계학술대회 및 국제심포지움 (2009.11)바로가기
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- 페이지
- pp.87-87
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A114446
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- Newcastle disease virus (NDV) is not only one of the most economically important pathogen
of poultry but also has a potential as anti-cancer virotherapy. To enhance the NDV vaccine
productivity, chicken fibroblast, DF-1 cell, was genetically engineered and rapid virus
quantification method was developed. V protein of NDV is well known for its interferon antagonistic activity. The addition of anti-interferon antibody increased NDV virus productivity in cell-based production system. Stable cell lines expressing either lentogenic or velogenic NDV V proteins were established. The overexpression of either lentogenic or velogenic V proteins had an effect of enhancing NDV production but did not increase the productivity of Japanese encephalitis virus (JEV). Our results indicate that NDV V protein have a function of interferon antagonist through inhibiting the increase of type-I interferons rather than incorporating virus particles as a structural protein or degrading STAT proteins. The interferon signals in NDV V-expressing cell lines were weakened by decreased activation or expression of dsRNA-activated enzymes, PKR and OAS. Interferon-antagonist activity seems to help rapid virus replication in the early phase of virus production. A method for rapid detection and quantification of NDV was developed. A SYBR Green I-based real-time RT-PCR was designed using a conventional, inexpensive RT-PCR kit targeting the F gene of the NDV LaSota strain. The method developed in this study was validated for specificity, accuracy, precision, linearity, limit of detection, limit of quantification, and robustness. The validation results satisfied the pre-determined acceptance criteria. The validated method was used to quantify virus samples produced in animal cell culture-based production system. Our results demonstrated that the real-time RT-PCR method is suitable for the rapid detection and quantification of whole virus particles irrespective of viral infectivity.
키워드
Newcastle disease virus (NDV) Vaccine production Interferon (IFN)-antagonist V protein Real-time RT-PCR virus quantification.저자
간행물 정보
발행기관
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- 발행기관명
- 한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
- 설립연도
- 1984
- 분야
- 공학>생물공학
- 소개
- 이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
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- 간행물명
- 한국생물공학회 학술대회
- 간기
- 반년간
- 수록기간
- 1985~2013
- 십진분류
- KDC 476 DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2009 추계학술대회 및 국제심포지움
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