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Biotron Bioreactor Symposium : Recent Advances and Application of Biomanufacturing Technology : 공동개최 : 바이오트론 : 좌장: 김익환 교수(고려대학교)
13C-Metabolic flux analysis and metabolic regulation analysis of E.coli for performance improvement of bioreactors
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- 발행기관
- 한국생물공학회 바로가기
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- 간행물
- 한국생물공학회 학술대회 바로가기
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- 통권
- 2009 추계학술대회 및 국제심포지움 (2009.11)바로가기
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- 페이지
- pp.87-87
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A114444
※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.
원문정보
초록
- 영어
- Extensive genetic modifications have been made to improve the performance of the bioreactor
systems. Although metabolic engineering has been paid much attention with some success in
practice, the pathway modifications based on the specific genes knockouts and/or amplifications
by plasmids do not necessarily lead to the significant improvement in the cell growth and/or the
specific metabolite production without profound insight into the metabolic regulation. Although
much information is available on genetic regulation, biochemistry, and physiology of cellular
metabolism, we are still far from understanding its overall regulation mechanism. It is strongly
desired that different levels of (omics) information be integrated by comparison of the data for
different mutants and/or the data obtained at different culture environments with the aid of
systems biology approach. Among the different levels of information, the metabolic flux
distribution is located on top of them, and it is quite important for understanding the whole cell
metabolism in practice. We have, so far, established the 13C-metabolic flux analysis method for
the continuous culture using NMR, GC-MS, and/further extended to the batch culture using CETOF/ MS. It was found that the cell metabolism significantly changes with respect to time for
specific gene knockout mutants as well as the wild type based on 13C-metabolic flux analysis.
We have also attempted to compare the flux data with other levels of information. We have also
investigated the effects of different carbon sources, nitrogen levels, phosphate levels,
temperature, and pH on the cell metabolism in view of global regulators and metabolic pathway
genes. Moreover, we are now developing a model which can simulate the metabolic changes
due to specific gene knockout. The goal of our research is to understand the whole cell
metabolism, and to realize its mechanism into the computer for the systematic cell design of
E.coli for the production of next generation bioenergy.
저자
간행물 정보
발행기관
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- 발행기관명
- 한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
- 설립연도
- 1984
- 분야
- 공학>생물공학
- 소개
- 이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다 1. 생물공학 분야의 발전을 위한 연구 협력 2. 생물공학의 실용화를 촉진시키기 위한 산학 협동 3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최 4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간 5. 생물공학 발전을 위한 정책 건의 6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동
간행물
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- 간행물명
- 한국생물공학회 학술대회
- 간기
- 반년간
- 수록기간
- 1985~2013
- 십진분류
- KDC 476 DDC 576
이 권호 내 다른 논문 / 한국생물공학회 학술대회 2009 추계학술대회 및 국제심포지움
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