We describe simultaneous assessments of bacterial phenotypes by using an integrated microfluidic system. The microfluidic system enables creating a series of different growth conditions, preserving growth media inside nanoliter reactors, and cultivating multiple bacteria with parallel nanoliter reactors. From a single on chip experiment, we assessed the effect of carbon source compositions on the bacterial phenotypes, i.e. the pattern of growth curves, with a wild Escherichia coli K-12 and lacZ knock-out mutant, as a model bacteria system. Diauxie growth curve, which is the stepwise response of bacteria to mixed carbon sources in a medium, was observed only from wild E. coli K-12. This study provides a new procedure for systematic analyses of phenotypes on effectors from single genotypes or multiple genotypes for microbial technology.
저자
Jihyune F. Kim [ Korea Research Institute of Bioscience and Biotechnology Department of Mechanical Engineering, Auburn University ]
Jong Wook Hong [ Korea Research Institute of Bioscience and Biotechnology Department of Mechanical Engineering, Auburn University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동