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Allergenicity Assessment of Cry Proteins in Insect-resistant Genetically Modified Maize Bt11, MON810, and MON863

첫 페이지 보기
  • 발행기관
    한국식품과학회 바로가기
  • 간행물
    Food Science and Biotechnology SCIE KCI 등재 SCOPUS 바로가기
  • 통권
    Volume 18 Number 5 (2009.10)바로가기
  • 페이지
    pp.1273-1278
  • 저자
    Jae-Hwan Kim, Young-Ju Seo, Ji-Young Kim, Young-Shin Han, Kwang-Shin Lee, Sun-Ah Kim, Han-Na Kim, Kangmo Ahn, Sang-II Lee, Hae-Yeong Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A112657

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원문정보

초록

영어
This study aimed to evaluate the potential allergenicity of Cry proteins in insect-resistant genetically modified (GM) maizes (Bt11, MON810, and MON863) using serum screening tests. Serum samples were obtained from Korean children (0-15 years old) with allergic symptoms who had positive maize-specific IgE. The levels of serum specific IgE was measured by the Phadia ImmunoCAP system and considered as positive when they are 0.35 kU/L or higher. Cry proteins (Cry1Ab in Bt11, mCry1Ab in MON810, and Cry3Bb1 in MON863) were expressed in Escherichia coli and purified for serum screening. The reactivity of purified Cry proteins was confirmed by IgE immunoblots in 50 patients (maize-sensitized patients). There was no reaction between Cry proteins and sera from maize-sensitized patients. Our results suggest that these Cry proteins are not likely to cause allergic reactions. Further studies using more sera from patients with true clinical allergies are needed to evaluate the potential allergenicity of novel proteins in GM maize.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

genetically modified maize Cry protein serum allergenicity immunoblot

저자

  • Jae-Hwan Kim [ Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University ]
  • Young-Ju Seo [ Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University ]
  • Ji-Young Kim [ Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine ]
  • Young-Shin Han [ Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine ]
  • Kwang-Shin Lee [ Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine ]
  • Sun-Ah Kim [ Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University ]
  • Han-Na Kim [ Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University ]
  • Kangmo Ahn [ Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine ]
  • Sang-II Lee [ Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine ]
  • Hae-Yeong Kim [ Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국식품과학회 [Korean Society of Food Science and Technology]
  • 설립연도
    1970
  • 분야
    농수해양>식품과학
  • 소개
    본회는 사회일반의이익에 공여하기 위하여 공공법인의 운영에 관한 법률의 규정에 따라 식품 과학 전반에 관한 이론 및 기술연구와 그 응용을 촉진 및 보급시키며 학술과 사업을 연계시키고, 나아가 국민의 식생활을 질적, 양적으로 향상시킴을 목적으로 한다.

간행물

  • 간행물명
    Food Science and Biotechnology
  • 간기
    격월간
  • pISSN
    1226-7708
  • 수록기간
    1992~2009
  • 십진분류
    KDC 574 DDC 664

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