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Developmental Characteristics of SCNT Pig Embryos Knocked-out of Alpha-1,3-Galactosyltransferase Gene

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 33 No 3 (2009.09)바로가기
  • 페이지
    pp.157-162
  • 저자
    Joo-Hyun Shim1, Mi-Rung Park, Byoung-Chul Yang, Yeoung-Gyu Ko, Keon Bong Oh, Jeong-Woong Lee, Jae-Seok Woo, Eung-Woo Park, Soo-Bong Park, Seongsoo Hwang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A111950

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원문정보

초록

영어
This study was performed to comprehend the developmental characteristics of cloned embryos knocked out (KO) of α-1,3-galactosyltransferase (GalT) gene. Immature oocytes were collected and cultured for 40 hrs (1-step) or 20hrs (with hormone) + 20hrs (without hormone) (2-step). The embryos transferred with miniature pig ear fibroblast cell were used as control. The reconstructed embryos were cultured in PZM-3 with 5% CO2 in air at 38.5℃ for 6 days. To determine the quality of the blstocysts, TUNEL and quantitative realtime RT-PCR were performed. The embryos were transferred to a surrogate (Landrace) at an earlier stage of the estrus cycle. The maturation rate was significantly higher in 2-step method than that of 1-step (p<0.05). The blastocyst development of GalT KO embryos was significantly lower than that of normal cloned embryos (p<0.05). The total and apoptotic cell number of GalT KO blastocysts was not different statistically from control. The relative abundance of Bax-α/Bcl-xl ratio was significantly higher in both cloned blastocysts than that of in vivo blastocysts (p<0.05). Taken together, it can be postulated that the lower developmental potential and higher expression of apoptosis related genes in GalT KO SCNT embryos might be a cause of a low efficiency of GalT KO cloned miniature pig production.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Chemicals
  In Vitro Maturation of Oocytes
  Nuclear Transfer Procedure
  Collection of In Vivo Blastocysts
  TUNEL Assay for Apoptotic Cell Death
  Apoptosis Related Gene Expression
  Statistical Analysis
 RESULTS
  In Vitro Maturation of Porcine Oocytes
  In Vitro Development of SCNT Embryos
  Apoptosis Rate in mNT and GalT KO NT Blastocysts
  Analysis of Apoptosis Gene Expression in Blastocysts
 DISCUSSION
 REFERENCES

키워드

3-Galactosyltransferase Gene knock out Miniature pig Embryo development Apoptosis α-1

저자

  • Joo-Hyun Shim1 [ National Institute of Animal Science, RDA ]
  • Mi-Rung Park [ National Institute of Animal Science, RDA ]
  • Byoung-Chul Yang [ National Institute of Animal Science, RDA ]
  • Yeoung-Gyu Ko [ National Institute of Animal Science, RDA ]
  • Keon Bong Oh [ National Institute of Animal Science, RDA ]
  • Jeong-Woong Lee [ Center for Regenerative Medicine, KRIBB ]
  • Jae-Seok Woo [ National Institute of Animal Science, RDA ]
  • Eung-Woo Park [ National Institute of Animal Science, RDA ]
  • Soo-Bong Park [ National Institute of Animal Science, RDA ]
  • Seongsoo Hwang [ National Institute of Animal Science, RDA ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 33 No 3

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