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Factors Affecting Primary Culture of Nuclear Transfer Blastocysts for Isolation of Embryonic Stem Cells in Miniature Pigs
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- 발행기관
- 한국동물생명공학회(구 한국동물번식학회) 바로가기
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- 간행물
- Reproductive & developmental biology 바로가기
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- 통권
- Volume 33 No 3 (2009.09)바로가기
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- 페이지
- pp.133-137
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A111946
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초록
- 영어
- Pluripotent embryonic stem (ES) cells isolated from inner cell mass (ICM) of blastocyst-stage embryos are capable of differentiating into various cell lineages and demonstrate germ-line transmission in experimentally produced chimeras. These cells have a great potential as tools for transgenic animal production, screening of newly-developed drugs, and cell therapy. Miniature pigs, selectively bred pigs for small size, offer several advantages over large breed pigs in biomedical research including human disease model and xenotransplantation. In the present study, factors affecting primary culture of somatic cell nuclear transfer blastocysts from miniature pigs for isolation of ES cells were investigated. Formation of primary colonies occurred only on STO cells in human ES medium. In contrast, no ICM outgrowth was observed on mouse embryonic fibroblasts (MEF) in porcine ES medium. Plating intact blastocysts and isolated ICM resulted in comparable attachment on feeder layer and primary colony formation. After subculture of ES-like colonies, two putative ES cell lines were isolated. Colonies of putative ES cells morphologically resembled murine ES cells. These cells were maintained in culture up to three passages, but lost by spontaneous differentiation. The present study demonstrates factors involved in the early stage of nuclear transfer ES cell isolation in miniature pigs. However, long-term maintenance and characterization of nuclear transfer ES cells in miniature pigs are remained to be done in further studies.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemicals and Reagents
In Vitro Maturation of Oocytes
Nuclear Transfer
Isolation of Inner Cell Mass
Preparation of Feeder Cells
Isolation of Embryonic Stem Cells
Statistical Analysis
RESULTS
Effects of Feeder Cells on Attachment of Blastocysts and Colony Formation
Effects of ICM Isolation on Their Attachment and Colony Formation
DISCUSSION
REFERENCES
키워드
Nuclear transfer Blastocyst Embryonic stem cells Miniature pig저자
간행물 정보
발행기관
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- 발행기관명
- 한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
- 설립연도
- 1976
- 분야
- 농수해양>축산학
- 소개
- 동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.
간행물
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- 간행물명
- Reproductive & developmental biology
- 간기
- 계간
- pISSN
- 1738-2432
- eISSN
- 2288-0151
- 수록기간
- 1977~2018
- 십진분류
- KDC 527 DDC 636
이 권호 내 다른 논문 / Reproductive & developmental biology Volume 33 No 3
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