Earticle

초록

영어

Production of the antioxidant substances by Bacillus polyfermenticus SCD was investigated using shake-flaskfermentation. The one-factor-at-a-time method was first employed to determine the key ingredients for optimal mediumcomposition, then further investigation of the medium composition was performed using response surface methodology(RSM). The antioxidant activity was measured using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assays. After screeningvarious elements, fructose, tryptone, and MgSO4·7H2O were chosen as the main factors for study in the statisticalexperimental design. Central composite design (CCD) was then used to determine the optimal concentrations of these 3components. Under the proposed optimized medium containing 2.8% fructose, 1.34% tryptone, 0.015% MgSO4·7H2O, 0.5%NaCl, and 0.25% K2HPO4, the model predicted an antioxidant activity of 80.5% (R2=0.9421). The actual experimental resultswere in agreement with the prediction.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

저자

• Jang-Hyun Lee [ Division of Animal Life Science, Konkuk University, Seoul 143-701, Korea ]
• Mi-Seung Chae [ Division of Animal Life Science, Konkuk University, Seoul 143-701, Korea ]
• Gooi Hun Choi [ Division of Animal Life Science, Konkuk University, Seoul 143-701, Korea ]
• Na-Kyoung Lee [ Division of Animal Life Science, Konkuk University, Seoul 143-701, Korea ]
• Hyun-Dong Paik [ Division of Animal Life Science, Konkuk University, Seoul 143-701, Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Food Science and Biotechnology

• 간기

  격월간

• pISSN

  1226-7708

• 수록기간

  1992~2009

• 십진분류

  KDC 574 DDC 664